From Wikipedia:
Amazon Prime is a paid subscription service offered by Amazon that gives users access to free two-day delivery (one-day in some areas) [...] and other benefits for a monthly or yearly fee. In April 2018, Amazon reported Prime had more than 100 million subscribers.
It's my understanding that express shipping like this involves shipping multiple smaller packages from various locations (instead of combining shipments), and sending things by airplane and small trucks or cars instead of slower ships, large trucks, or trains. It seems clear that all of these changes result in increased greenhouse gas (GHG) emissions compared to standard shipping.
Is there any research or analysis on how much increase in GHG emissions is caused by Amazon Prime? Obviously some assumption must be made about how often Prime users would opt for express shipping were Prime not available.